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Objective To investigate the changes of serum concentration of amylase(AMY) and pathological of pancreas in severe acute pancreatitis of SD-rats treated with Ulinastatin combined with qingyitang and to provide the basis for clinical application.Methods Ninty male SD-rats with average weight of(271.59±11.13) g were conventional feeding for a week.Twelve hours before experiment,they were forbidden to take food.They were randomly divided into five groups(with 18 in every group):sham operation group,severe acute pancreatitis group,Ulinastatin treated group,qingyitang treated group,Ulinastatin combined with qingyitang treated group.Then eighteen rats in every group were divided randomly into 6 h,12 h and 24 h groups with six rats in each group.Severe acute pancreatitis model was created by retrograde injection of 5％ sodium taurocholate (0.1 ml/100 g) through pancreatic duct,in sham operation group,only pancreas were flipped.The three treated groups were treated with vein injection of Ulinastatin(20 000 U/kg)or qingyitang perfusion(1 ml/100 g)or Ulinastatin combined with qingyitangat 0 h,5 h and 11 h after severe acute pancreatitis model successfully created.AMY and patholigical changes of pancreas were measured.Results There was significant difference on AMY level at 6 h,12 h and 24.h between severe acute pancreatitis group and sham operation group[6 h:(16 518±327)U/L vs(1671±175)U/L,F =3296.24,P ＜0.01;12 h:(16 796±338) U/L vs(1751±223)U/L,F =3802.44,P ＜0.01;24 h:(1554±373) U/L vs(1724±187) U/L,F =2382.54,P ＜0.01].Compared with severe acute pancreatitis group,the AMY level and pathologic score both decreased significantly in Ulinastatin treated group,qingyitang treated group and Ulinastatin combined with qingyitang treated group(P ＜0.05 or P ＜ 0.01).At the same time,the AMY level and pathologic score both decreased more in Ulinastatin combined with qingyitang treated group than Ulinastatin treated group and qingyitang treated group(P ＜0.05 or P ＜ 0.01).Conclusion Combination of Ulinastatin with qingyitang in treatment of severe acute pancreatitis of rats is superior to treatment with single medicine.
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Objective To investigate the efficacy and safety of Fuzheng huayu capsule in combination With anti-viral drugs in the treatment of hepatitis B-mediated liver cirrhosis.Methods One hundred and twentysix patients with liver cirrhosis resulting from hepatitis B were randomized into group A (38 cases receiving conventional therapy),group B (41 cases receiving conventional therapy + entecavir)and group C (47 cases receiving conventional therapy + entecavir and anti-fibrosis treatment ).The Fuzheng Huayu capsule was administrated for 24 weeks and all the patients were observed for 48 weeks.Results The liver cirrhosis-related parameters in Group C were more remarkably improved than that of Group A and Group B:HA( [ 152.3 ± 72.3 ]μg/L vs.[212.3 ± 86.9] μg/L,[325.6 ± 153.1 ] μg/L,F =30.18,P ＜ 0.01 ),LN( [ 104.7 ± 23.9 ] μg/L vs.[ 139.9 ±28.9] μg/L,[ 127.7 ±76.0] μg/L,F =36.99,P ＜0.01 ),Pc Ⅲ ( [ 167.8 ±61.4] μg/L vs.[207.5 ±78.1 ] μg/L,[ 263.1 ± 113.2 ] μg/L,F =30.34,P ＜ 0.01 ) and Child-Pugh scale ( [ 6.94 ± 1.31 ] vs.[ 7.53 ±1.24 ],[ 8.77 ± 1.36 ],F =14.45,P ＜ 0.01 ).The liver function-related parameters in Group C were significantly different from that of Group A and Group B:ALT( [58 ±41 ] U/L vs.[ 147 ±96] U/L,[75 ± 19]U/L,F =16.82,P ＜0.01 ),ALB( [38.1 ± 1.7]g/L vs.[26.5 ±3.5 ] g/L,[35.4 ± 1.8 ] g/L,F =4.69,P ＜0.01),TBil ([31.9 ± 12.7] μmol/L vs.[85.2 ±58.3] μmol/L,[46.1 ± 17.8] μmol/L,F.=15.10,P ＜0.01)and PTA improvement ([76 ±24]％ vs.[57 ± 12]％,[73 ± 18]％,F =79.26,P ＜0.01).Conclusion Entecavir can rapidly and effectively inhibit the replication of HBV in patients with decompensated hepatitis Bmediated cirrhosis and improve the liver function.When combined with fuzheng huayu capsule,entecavir will effectively improve the cirrhosis and elevate the serum albumin level.
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Objective To compare the curative effect of magnesium isoglycyrrhizinate and compound ammonium glycyrrhetate in patients with liver cirrhosis of decompensated.Methods Eighty-six patients with liver cirrhosis of decompensated were enrolled into the study and randomly divided into two groups: the treatment group(n=43) were given magnesium isoglycyrrhizinate once a day in addition to routine treatment;the control group(n=43) were given compound ammonium glycyrrhetate once a day in addition to routine treatment.The clinical manifestation,including symptoms,signs and hepatic function were observed.The clinical lab data,including blood routine examination,urine routine test and kidney function of all patients from two groups were collected before and after the treatments.The adverse drug reactions were monitored throughout the whole therapy.Results ALT and AST turned to normal in 97.7%(42/43) and 90.7%(39/43) of patients respectively in the treatment group,which were significantly higher than those of 72.1%(31/43) and 74.4%(32/43) respectively in the control group( x2=9.86 and 4.73,respectively,Ps＜0.05). Time to turning to normal in ALT and AST were(21.6±9.1)d and(23.1±10.6)d in the treatment group,which were significantly lower than those of(37.5±17.8)d,and(46.7±19.4)d respectively in the control group(t=5.23 and 7.01,respectively,Ps＜0.01).Conclusion The results suggested that magnesium isoglycyrrhizinate had better effect on alleviating symptoms and decreasing enzyme in treatment of liver cirrhosis of decompensated stage.
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Objective To evaluate the anti-fatigue effect by taurine in mice.Methods Mice were randomly divided into three groups,two groups were given taurine at dose of 10.0 g/L and 2.5 g/L respectively,and the control group was given physiological Saline.After 30 days of intervention,the swimming test was carried out,the liver glycogen,serum BUN and Lactic acid were also measured.Results Both high taurine and low taurine prolonged the swimming time significantly comparing with the control group ([380.9 ± 65.5 ] s vs [226.0 ± 44.8 ] s vs [175.7 ± 24.4 ] s,P ＜ 0.05 ).Liver glycogen also increased significantly,they were ( 21.85 ± 4.21 ),( 13.26 ± 2.16 ) ( 7.13 ± 1.34 ) mg/g in high,low taurine and control group respectively.The level of BUN and LAC were decreased significantly in taurine groups ( P ＜ 0.05 ).Conclusion Taurine may has the anti-fatigue effect to some extent.